The Rattlesnake Hills AVA is located in Yakima County. The Rattlesnake Hills AVA is entirely contained within the Yakima Valley AVA, which is in turn is entirely contained within the larger Columbia Valley AVA. The hills form the northern boundary of Yakima Valley, and the AVA includes land between the north bank of the Sunnyside Canal and the entirety of the southern slopes of the Rattlesnake Hills between Outlook and the Wapato Dam. The AVA is centered on the city of Zillah. With elevations ranging from 850 feet (259 m) to 3,085 feet (940 m), this AVA contains the highest point in the Yakima Valley AVA.

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